#TOPCAT COM SOFTWARE#

When an internal segment fails to map - again suggesting that such Least 45bp) are mapped at a certain distance on the same genomic Junction is when two segments from the same read (for reads of at The first and strongest source of evidence for a TopHat generates its database of possible splice junctions from two Program to produce the end-to-end read alignments. Segment alignments are put back together in a final step of the Reads into smaller segments which are then mapped independently. TopHat solves this problem mainly by splitting all input Longer but many exons are shorter than this so they would be missed in Short read sequencing machines can currently produce reads 100bp or

Junctions and then maps the reads against these junctions to confirm Using this initial mapping information, TopHat builds a database of TopHat can find splice junctions without a reference annotation.Р”Р‡irst mapping RNA-Seq reads to the genome, TopHat identifies potentialР”В®xons, since many RNA-Seq reads will contiguously align to the genome.

#TOPCAT COM SOFTWARE#

The software is optimized for reads 75bp or longer. In TopHat 1.1.0, we began supporting Applied Biosystems' Colorspace format. TopHat was designed to work with reads produced by the Illumina GenomeР”Р„nalyzer, although users have been successful in using TopHat with readsР”Р‡rom other technologies. What types of reads can I use TopHat with? TopHat is a program that aligns RNA-Seq reads to a genome in order

Topcat com